WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-FREE1/ Protein FREE1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT1G20110，Q9ASS2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | AT1G20110 |
|---|---|
| Alternative Names | FREE1 FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 FYVE-DOMAIN PROTEIN 1 FYVE1 PDE330 PIGMENT DEFECTIVE EMBRYO 330 |
| Molecular Weight(MW) | 65.4 kDa for Arabidopsis |
| Function | FREE1 encodes a protein that is localized to the peripheral membrane of late endosomal compartments. It is involved in the regulation of mulitivesicular/prevacuolar compartment protein sorting. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
