WB
Loading: Serially diluted antigen Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-FVE/ WD-40 repeat-containing protein MSI4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT2G19520，O22607 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
| Gene Symbol | AT2G19520 |
|---|---|
| Alternative Names | ACG1 ATMSI4 FVE MSI4 MULTICOPY SUPPRESSOR OF IRA1 4 NFC04 NFC4 |
| Molecular Weight(MW) | 55.8 kDa for Arabidopsis |
| Function | FVE is involved in RNA-directed DNA methylation by facilitating the recruitment of Pol V to chromatin. It is an important component of the flowering autonomous pathway which positively regulates flowering by promoting transcriptional repression of the flowering repressor FLC. |
WB
Loading: Serially diluted antigen Electrophoresis: 10% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
