WB
Loading: Figure 1 — Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Figure 2 — Serially diluted antigen protein Electrophoresis: Figure 1 — 12.5% Tricine gel; Figure 2 — 10% SDS gel Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-FRO1/ NADH dehydrogenase [ubiquinone] iron-sulfur prote |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT5G67590，Q9FJW4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT5G67590 |
|---|---|
| Alternative Names | FRO1 FROSTBITE1 NADH:UBIQUINONE OXIDOREDUCTASE FE-S PROTEIN4 NDUFS4 |
| Molecular Weight(MW) | 17 / 14.5 kDa for Arabidopsis and Rice |
| Function | FRO1 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I)，that functions in the transfer of electrons from NADH to the respiratory chain. |
WB
Loading: Figure 1 — Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves Figure 2 — Serially diluted antigen protein Electrophoresis: Figure 1 — 12.5% Tricine gel; Figure 2 — 10% SDS gel Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
