WB
Loading: Approximately 2 μg total protein from rapeseed and rice leaves Electrophoresis: 12.5% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GLB1/ Nitrogen regulatory protein P-II homolog |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT4G01900, Q9ZST4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | RiceBrassica napus |
| Gene Symbol | AT4G01900 |
|---|---|
| Alternative Names | GLB1 GLNB1 HOMOLOG PII |
| Molecular Weight(MW) | 21 / 15 kDa forBrassica napusL. and Rice |
| Function | GLB1 encodes a PII protein that may function as part of a signal transduction network involved in perceiving the status of carbon and organic nitrogen. It forms a protein complex with N-acetylglutamate kinase and regulates the kinase activity by relieving the feedback inhibition of the kinase by arginine. |
WB
Loading: Approximately 2 μg total protein from rapeseed and rice leaves Electrophoresis: 12.5% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
