WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-F3H/ Flavanone 3-hydroxylase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT3G51240 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Gene Symbol | AT3G51240 |
|---|---|
| Alternative Names | F3'H Naringenin 3-dioxygenase Protein TRANSPARENT TESTA 6 TT6 Naringenin 2-oxoglutarate 3-dioxygenase |
| Molecular Weight(MW) | 40 / 38 kDa forArabidopsis |
| Function | F3H encodes flavanone 3-hydroxylase that is coordinately expressed with chalcone synthase and chalcone isomerases and is involved in flavonoid biosynthesis. |
WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
