WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-WHY3/ Single-stranded DNA-binding protein WHY3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT2G02740 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT2G02740 |
|---|---|
| Alternative Names | PTAC11 ATWHY3 PLASTID TRANSCRIPTIONALLY ACTIVE11 WHIRLY 3 |
| Molecular Weight(MW) | 29 / 21 kDa forArabidopsis |
| Function | Single-stranded DNA-binding protein that functions in both chloroplasts and nucleus. In chloroplasts, it maintains plastid genome stability by preventing break-induced and short homology-dependent illegitimate recombinations. In the nucleus, it is recruited to a distal element upstream of the kinesin KP1 to mediate the transcriptional repression of KP1. |
WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS‑PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
