WB
Loading: 2 μg total protein from Arabidopsis thaliana / rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-SHM1 / Serine hydroxymethyltransferase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana AT4G37930. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT4G37930, LOC_Os03g52840 |
|---|---|
| Alternative Names | SHM1 Serine hydroxymethyltransferase 1 serine transhydroxymethyltransferase serine transhydroxymethyltransferase 1 SHMT1 STM. |
| Molecular Weight(MW) | 52 / 57 kDa forArabidopsis thaliana52 / 56 kDa for rice |
| Function | Serine hydroxymethyltransferases (SHMs) are important enzymes of cellular one-carbon metabolism and are essential for the photorespiratory glycine-into-serine conversion in leaf mesophyll mitochondria. SHM1 catalyzes the conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. |
WB
Loading: 2 μg total protein from Arabidopsis thaliana / rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:5000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
