WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-VHA-A/V-type proton ATPase catalytic subunit A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana AT1G78900 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT1G78900 |
|---|---|
| Alternative Names | V-ATPase subunit A |
| Molecular Weight(MW) | 69 kDa forArabidopsis thalianaand Rice |
| Function | The catalytic subunit A of the vacuolar ATP synthase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. |
WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skim milk, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
