WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-GSH1/ Glutamate--cysteine ligase Rabbit Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana AT4G23100 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT4G23100 |
|---|---|
| Alternative Names | ATECS1 ATGSH1 CAD2 CADMIUM SENSITIVE 2 GSHA PAD2 |
| Molecular Weight(MW) | 58.6 / 49 kDa forArabidopsis thaliana |
| Function | Glutamate-cysteine ligase (GSH1) catalyzes the first, and rate-limiting, step of glutathione biosynthesis. It is required for cell proliferation at the root tip, and involved in susceptibility to the bacterial pathogen Pseudomonas syringae. |
WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
