WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-Cyt b6 / PetB / Cytochrome b6 of Cyt b6/f antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ATCG00720 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | ATCG00720, LOC_Os10g21324 |
|---|---|
| Alternative Names | PetB Cytochrome b6/f complex Cyt b6 Photosynthetic electron transfer B. |
| Molecular Weight(MW) | 24/24 kDa forArabidopsis thaliana26/24 kDa for Rice |
| Function | The cytochrome b(6) subunit is the component of the cytochrome b6f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. |
WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
