WB
Loading: 2 μg total protein from Arabidopsis thaliana / rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Product Name | Anti-CAC3 / carboxyltransferase alpha subunit of acetyl-CoA |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana AT2G38040. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT2G38040 |
|---|---|
| Alternative Names | Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha CAC3. |
| Molecular Weight(MW) | 85 / 80 kDa forArabidopsis thaliana |
| Function | Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO2 group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA |
WB
Loading: 2 μg total protein from Arabidopsis thaliana / rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted at 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:5000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
