WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-APL1/ADG2 rabbit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana At5g19220 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | At5g19220, LOC_Os03g52460 |
|---|---|
| Alternative Names | ADG2 Glucose-1-phosphate adenylyltransferase large subunit 1 ADP-glucose synthase |
| Molecular Weight(MW) | 58/52 kDa forArabidopsis thaliana |
| Function | APL1 catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP, and plays a role in the synthesis of starch. |
WB
Loading: Approximately 2 μg total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12.5% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
