WB
Loading: 1 μg total protein from Arabidopsis thaliana / rice leaves
Electrophoresis: 12.5% SDS-PAGE
Transfer: NC (nitrocellulose) membrane, 10 V overnight
Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h
Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h