WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves, together with serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-CPN20/ 20 kDa chaperonin, chloroplastic |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G20720，O65282 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT5G20720 |
|---|---|
| Alternative Names | ATCPN21 CHAPERONIN 20 CHCPN10 CHLOROPLAST CHAPERONIN 10 CPN10 CPN20 CPN21 |
| Molecular Weight(MW) | 26.8 / 21.3 kDa forArabidopsis thaliana |
| Function | CPN20 seems to function only as a co-chaperone, along with CPN60. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis thaliana and rice leaves, together with serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
