WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-ECA1/ Calcium-transporting ATPase 1, endoplasmic reticulum-type |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G07810，P92939 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT1G07810 |
|---|---|
| Alternative Names | ACA3 ARABIDOPSIS THALIANA ER-TYPE CA2+-ATPASE 1 ATECA1 ECA1 ER-TYPE CA2+-ATPASE 1 |
| Molecular Weight(MW) | 116 kDa forArabidopsis thaliana |
| Function | ECA1 encodes an ER-type Ca2+-pumping ATPase. It catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the endoplasmic reticulum lumen. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
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| Form | Liquid |
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| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
