WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted 1:5000, incubated at room temperature for 1 h| Product Name | Anti-TOPP1/ Serine/threonine-protein phosphatase PP1 isozyme 1 |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT2G29400，P30366 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT2G29400 |
|---|---|
| Alternative Names | PP1-AT PROTEIN PHOSPHATASE 1 TOPP1 TYPE ONE PROTEIN PHOSPHATASE 1 |
| Molecular Weight(MW) | 36.3 kDa forArabidopsis thaliana |
| Function | TOPP1 is Type 1 protein phosphatase, expressed in roots, rosettes and flowers. It acts as a negative regulator of abscisic acid (ABA) signaling. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: Diluted 1:5000, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
