WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-VHA-B/ V-type proton ATPase subunit B |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G76030，P11574 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT1G76030，AT1G20260，AT4G38510 |
|---|---|
| Alternative Names | ATVAB1 V-ATPASE B SUBUNIT 1 VAB1，VAB2，VAB3 VHA-B1，VHA-B2，VHA-B3 |
| Molecular Weight(MW) | 54 kDa forArabidopsis thaliana |
| Function | There are three genes encoding the vacuolar ATP synthase subunit B in Arabidopsis. These subunit proteins involved in actin filaments and plant development. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
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| Form | Liquid |
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| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
