WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-CRT2/ Calreticulin-2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G09210，Q38858 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT1G09210 |
|---|---|
| Alternative Names | AtCRT1b CALRETICULIN 2 CRT1b CRT2 calreticulin 1b |
| Molecular Weight(MW) | 48.2 kDa forArabidopsis thaliana |
| Function | CRT2 encodes one of three Arabidopsis calreticulins. It post-transcriptionally regulates together with CRT1 VAMP721/722 levels under ER stress. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
