WB
Loading: Approximately 3 μg total protein from Arabidopsis leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-CCD1/ Carotenoid Cleavage Dioxygenase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT3G63520，O65572 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Gene Symbol | AT3G63520 |
|---|---|
| Alternative Names | ATCCD1 ATNCED1 CCD1 NCED1 Carotenoid 9 10(9' 10')-cleavage dioxygenase 1 |
| Molecular Weight(MW) | 60.9 kDa forArabidopsis thaliana |
| Function | CCD1 encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. |
WB
Loading: Approximately 3 μg total protein from Arabidopsis leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
