WB
Loading: Serially diluted antigen protein and approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-PREP1/ Presequence protease 1, chloroplastic/mitochondrial |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT3G19170，Q9LJL3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Gene Symbol | AT3G19170 |
|---|---|
| Alternative Names | ATZNMP PREP1 PRESEQUENCE PROTEASE 1 presequence protease 1；PREP2 presequence protease 2 |
| Molecular Weight(MW) | 121 / 112 kDa forArabidopsis thaliana |
| Function | PREP1 is expressed only in siliques and flowers. It is ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage. |
WB
Loading: Serially diluted antigen protein and approximately 3 μg total protein from Arabidopsis and rice leaves Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
