WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Product Name | Anti-LINC1/ Protein LITTLE NUCLEI 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G67230，F4HRT5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 |
| Gene Symbol | AT1G67230 |
|---|---|
| Alternative Names | (JAPANESE FOR NUCLEUS) 2 ATLINC1 CROWDED NUCLEI 1 CRWN1 KAKU2 LINC1 LITTLE NUCLEI1 |
| Molecular Weight(MW) | 129 kDa forArabidopsis thaliana |
| Function | LINC1 encodes a nuclear coiled-coil protein related to the carrot peripheral nuclear protein NMCP1, that is involved in the determination of plant nuclear structure. Member of a small gene family in Arabidopsis contains 4 proteins (LNC1-4 or CRWN 1-4) with redundant functions in protection from oxidative damage, control of nuclear morphology and degradation of ABI5. |
WB
Loading: Serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Transfer: NC (nitrocellulose) membrane, 10 V overnight Blocking: 5% skimmed milk powder, incubated at room temperature for 1 h Primary antibody: 1:5000 dilution, incubated at room temperature for 1 h| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
