WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.IHC
Human placenta was stained with anti-MYPT1 rabbit antibodyICC/IF
Immunofluorescence analysis of MYPT1 Rabbit mAb. Picture A: MYPT1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Product Name | MYPT1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPP1R12A |
|---|---|
| Gene Synonyms | MBS GUBS M130 MYPT1 |
| Gene Full Name | protein phosphatase 1 regulatory subunit 12A |
| Gene Summary | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| Alternative Names | PPP1R12A MBS MYPT1 Protein phosphatase 1 regulatory subunit 12A Myosin phosphatase-targeting subunit 1 Myosin phosphatase target subunit 1 Protein phosphatase myosin-binding subunit |
| Molecular Weight(MW) | 115 kDa(Observed band size: 140 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
IHC
Human placenta was stained with anti-MYPT1 rabbit antibody
ICC/IF
Immunofluorescence analysis of MYPT1 Rabbit mAb. Picture A: MYPT1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
