WB
Western blot analysis of extracts of various cell lines, using SLC7A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 180s.IHC
Immunohistochemistry of paraffin-embedded human mammary cancer using SLC7A2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.IHC
Immunohistochemistry of paraffin-embedded human placenta using SLC7A2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.IHC
Immunohistochemistry of paraffin-embedded rat ovary using SLC7A2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.| Product Name | SLC7A2 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of human SLC7A2(NP_001008539.3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC7A2 |
|---|---|
| Gene Synonyms | CAT2 ATRC2 HCAT2 CAT-2A CAT-2B SLC7A2A SLC7A2B |
| Gene Full Name | solute carrier family 7 member 2 |
| Gene Summary | The protein encoded by this gene is a cationic amino acid transporter and a member of the APC (amino acid-polyamine-organocation) family of transporters. The encoded membrane protein is responsible for the cellular uptake of arginine, lysine and ornithine. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Molecular Weight(MW) | 72 KDa |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of extracts of various cell lines, using SLC7A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 180s.
IHC
Immunohistochemistry of paraffin-embedded human mammary cancer using SLC7A2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
IHC
Immunohistochemistry of paraffin-embedded human placenta using SLC7A2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
IHC
Immunohistochemistry of paraffin-embedded rat ovary using SLC7A2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
