Immunofluorescence
Sample Type: hRetinal pigment epithelial cellsGreen: primaryRed: nuclearPrimary Dilution: 1:200Secondary Antibody: goat anti-rabbit-Alexa 488Secondary Dilution: 1:500Image Submitted by: Brian KennedyIndiana University Northwest See Customer Feedback tab for detailed information.Western blot
Sample Type: 1-4. hRetinal pigment epithelial cells, 4 individual donors (20ug)Primary Dilution: 1:1000Secondary Antibody: goat anti-rabbit-APSecondary Dilution: 1:2000Image Submitted by: Brian KennedyIndiana University Northwest See Customer Feedback tab for detailed information.Western blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Product Name | Rabbit anti-TRPV5 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAT2, ECAC1, OTRPC3 |
| Immunogen | The immunogen for anti-TRPV5 antibody: synthetic peptide directed towards the N terminal of human TRPV5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRPV5 |
|---|---|
| Gene Synonyms | CAT2 ECAC1 OTRPC3 |
| Gene Full Name | Transient receptor potential cation channel, subfamily V, member 5 |
| Gene Summary | TRPV5 is a member of the transient receptor family and the TrpV subfamily. TRPV5, a calcium-selective channel, has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. TRPV5 forms homotetramers or heterotetramers and is activated by a low internal calcium level.This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2486 AF304464.1 86-2571 |
| Alternative Names | CAT2 ECAC1 OTRPC3 |
| Molecular Weight(MW) | 82kDa |
| Sequence | 729 amino acids |
Immunofluorescence
Sample Type: hRetinal pigment epithelial cellsGreen: primaryRed: nuclearPrimary Dilution: 1:200Secondary Antibody: goat anti-rabbit-Alexa 488Secondary Dilution: 1:500Image Submitted by: Brian KennedyIndiana University Northwest See Customer Feedback tab for detailed information.
Western blot
Sample Type: 1-4. hRetinal pigment epithelial cells, 4 individual donors (20ug)Primary Dilution: 1:1000Secondary Antibody: goat anti-rabbit-APSecondary Dilution: 1:2000Image Submitted by: Brian KennedyIndiana University Northwest See Customer Feedback tab for detailed information.
Western blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
