ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBTB7B antibody at dilution of 1:1600 and 488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).IP
IP result of anti-ZBTB7B (4ug; Detection: 1:3000) with HeLa cells lysate 1320 ug.WB
Various lysates were subjected to SDS PAGE followed by western blot with ZBTB7B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.| Product Name | ZBTB7B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ZBTB7B protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:1000-1:4000 ICC/IF:1:800-1:3200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZBTB7B |
|---|---|
| Gene Synonyms | vGAF CKROX THPOK ZFP67 ZBTB15 ZFP-67 c-KROX hcKROX ZNF857B |
| Gene Full Name | zinc finger and BTB domain containing 7B |
| Gene Summary | This gene encodes a zinc finger-containing transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. It also functions as a transcriptional repressor of type I collagen genes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Alternative Names | ZFP67 Zfp 67 ZBTB15 THPOK hcKrox |
| Molecular Weight(MW) | 60-65 kDa; 58 kD(Calculated). |
| Cellular Localization | Nucleus. |
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBTB7B antibody at dilution of 1:1600 and 488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IP
IP result of anti-ZBTB7B (4ug; Detection: 1:3000) with HeLa cells lysate 1320 ug.
WB
Various lysates were subjected to SDS PAGE followed by western blot with ZBTB7B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.| Application Notes | WB:1:1000-1:4000 ICC/IF:1:800-1:3200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
