WB
Western blot analysis of TRA-1-81 expression in total cell lysates of ECV304 (A), Raji (B), MIA PaCa-2 (C), HeLa (D), Jurkat (E), and NTERA-2 cl. D1 (F).WB
Western blot analysis of TRA-1-81 (A) and TRA-1-60 (B) expression in NTERA-2 cl. D1 whole-cell lysate. The antibodies used for detection include TRA-1-81 antibody (TRA-1-80)(A) and TRA-1-60 (TRA-1-60)(B).WB
TRA-1-81 Antibody (TRA-1-80): Western blot analysis of TRA-1-81 expression in PC-3 whole-cell lysate.FC
Stain NTERA-2 cl. D1 cells with TRA-1-81 antibody (TRA-1-80), and then perform indirect FCM analysis using PE-conjugated goat anti-mouse IgM. The quadrant labeling was set according to the isotype control normal mouse IgM.FC
Indirect FCM analysis of live NTERA-2 cl. D1 cells stained with TRA-1-81 antibody (TRA-1-80), then stained with PE-conjugated goat anti-mouse IgM. Quadrant labeling was set according to the isotype control normal mouse IgM.ICC/IF
Immunofluorescence staining of methanol-fixed NTERA-2 cl. D1 cells, showing membrane localization.WB
Western blot analysis of TRA-1-81 expression in total cell lysates of untreated K-562 (A), chemically treated K-562 (B), untreated HCT-116 (C), and chemically treated HCT-116 (D). The reagents used: m-IgGκ BP-HRP. β-Actin (C4), used as a loading control. Reagents used: m-IgG Fc BP-HRP.| Product Name | Mouse Monoclonal antibody to TRA-1-81(TRA-1-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against 2102Ep human embryonal carcinoma cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM,k |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIPWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 FC(1 µg per 1 x 106 cells) |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PODXL |
|---|---|
| Gene Synonyms | PC PDX PCLP Gp200 gp135 PCLP-1 PODXL1 |
| Gene Full Name | podocalyxin like |
| Gene Summary | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
WB
Western blot analysis of TRA-1-81 expression in total cell lysates of ECV304 (A), Raji (B), MIA PaCa-2 (C), HeLa (D), Jurkat (E), and NTERA-2 cl. D1 (F).
WB
Western blot analysis of TRA-1-81 (A) and TRA-1-60 (B) expression in NTERA-2 cl. D1 whole-cell lysate. The antibodies used for detection include TRA-1-81 antibody (TRA-1-80)(A) and TRA-1-60 (TRA-1-60)(B).
WB
TRA-1-81 Antibody (TRA-1-80): Western blot analysis of TRA-1-81 expression in PC-3 whole-cell lysate.
FC
Stain NTERA-2 cl. D1 cells with TRA-1-81 antibody (TRA-1-80), and then perform indirect FCM analysis using PE-conjugated goat anti-mouse IgM. The quadrant labeling was set according to the isotype control normal mouse IgM.
FC
Indirect FCM analysis of live NTERA-2 cl. D1 cells stained with TRA-1-81 antibody (TRA-1-80), then stained with PE-conjugated goat anti-mouse IgM. Quadrant labeling was set according to the isotype control normal mouse IgM.
ICC/IF
Immunofluorescence staining of methanol-fixed NTERA-2 cl. D1 cells, showing membrane localization.
WB
Western blot analysis of TRA-1-81 expression in total cell lysates of untreated K-562 (A), chemically treated K-562 (B), untreated HCT-116 (C), and chemically treated HCT-116 (D). The reagents used: m-IgGκ BP-HRP. β-Actin (C4), used as a loading control. Reagents used: m-IgG Fc BP-HRP.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 FC(1 µg per 1 x 106 cells) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
