WB
Various lysates were subjected to SDS PAGE followed by western blot with SETMAR antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.IP
IP result of anti-SETMAR (4ug; Detection:1:500) with HL-60 cells lysate 1600ug.WB
HL-60 cells were subjected to SDS PAGE followed by western blot with SETMAR Antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.| Product Name | SETMAR Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SETMAR protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:500-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Purification | Affinity purified |
| Gene Symbol | SETMAR |
|---|---|
| Gene Synonyms | Mar1 METNASE |
| Gene Full Name | SET and mariner transposase domain methyltransferase |
| Gene Summary | This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
| Alternative Names | HsMar1 METNASE SETMAR |
| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Chromosome;Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SETMAR antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IP
IP result of anti-SETMAR (4ug; Detection:1:500) with HL-60 cells lysate 1600ug.
WB
HL-60 cells were subjected to SDS PAGE followed by western blot with SETMAR Antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.| Application Notes | WB:1:500-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
