WB
WB result of VCP antibody (1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells.WB
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with VCP Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (Clone: 2A4B10 ) at dilution of 1:800 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).FC
1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (Clone:2A4B10) and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).| Product Name | VCP Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human VCP protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:400-1:1600 IHC:1:500-1:2000 FC:(INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | VCP |
|---|---|
| Gene Synonyms | p97 TERA CDC48 FTDALS6 |
| Gene Full Name | valosin containing protein |
| Gene Summary | This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. [provided by RefSeq, Aug 2017] |
| Alternative Names | 15S Mg(2+) ATPase p97 subunit 15S Mg(2+)-ATPase p97 subunit 2A4B10 EC:3.6.4.6 HEL-220 |
| Molecular Weight(MW) | 89 kDa(Observed Molecular Weight:89 kDa) |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum,Nucleus. |
WB
WB result of VCP antibody (1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells.
WB
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with VCP Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using VCP antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using VCP antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (Clone: 2A4B10 ) at dilution of 1:800 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC
1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (Clone:2A4B10) and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).| Application Notes | WB:1:500-1:2000 ICC/IF:1:400-1:1600 IHC:1:500-1:2000 FC:(INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
