WB
Various lysates were subjected to SDS PAGE followed by western blot with c-SRC antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with c-SRC antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using c-SRC antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using c-SRC Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using c-SRC Antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | c-SRC Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human c-SRC protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:100-1:400 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HamsterHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SRC |
|---|---|
| Gene Synonyms | ASV SRC1 THC6 c-SRC p60-Src |
| Gene Full Name | SRC proto-oncogene, non-receptor tyrosine kinase |
| Gene Summary | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | SRC 5E10C4 c SRC cSRC EC:2.7.10.2 |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Cytoskeleton,Membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with c-SRC antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with c-SRC antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using c-SRC antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using c-SRC Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using c-SRC Antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:5000-1:50000 IHC:1:100-1:400 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
