WB
Various lysates were subjected to SDS PAGE followed by western blot with MLKL antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with MLKL antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using MLKL antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using MLKL antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using MLKL antibody at dilution of 1:100 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using MLKL antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).| Product Name | MLKL Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MLKL protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:50000 ICC/IF:1:400-1:1600 IHC:1:500-1:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | MLKL |
|---|---|
| Gene Synonyms | hMLKL |
| Gene Full Name | mixed lineage kinase domain like pseudokinase |
| Gene Summary | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | 3D4C6 hMLKL Mixed lineage kinase domain-like protein |
| Molecular Weight(MW) | 54 kDa(Observed Molecular Weight:35-40 kDa, 50-55 kDa) |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with MLKL antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with MLKL antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using MLKL antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using MLKL antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using MLKL antibody at dilution of 1:100 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using MLKL antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).| Application Notes | WB:1:5000-1:50000 ICC/IF:1:400-1:1600 IHC:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
