WB
Western blot analysis of extracts from A549 cells(UV treatment), using AE2 Antibody. The lane on the left was treated with blocking peptide.WB
AE2 was up-regulated in human ovarian cancer tissues. (A) AE2 mRNA was over-expressed in ovarian cancer tissues compared to the normal tissues in TCGA dataset. Box-whiskers plot (Min to Max) was shown. (B) Genomic alterations of AE1 (SLC4A1), AE2 (SLC4A2) and AE3 (SLC4A3) in cBioportal ovarian cancer database. (C) mRNA expression of AE2 was examined by qPCR and normalized to GAPDH expression in 100 pairs of matched ovarian cancer tissues and adjacent non-tumorous tissue. Box-whiskers plot (Min to Max) was shown. (D) The protein levels of AE2 were higher in 10 pairs of matched samples of ovarian cancer tissue (T), compared with adjacent non-tumorous tissues (N). GAPDH was served as a loading control. (E) Kaplan–Meier survival analysis of 100 patients with ovarian cancer.| Product Name | AE2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human AE2, corresponding to a region within the internal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-3000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC4A2 |
|---|---|
| Gene Synonyms | AE2 HKB3 BND3L NBND3 OPTB9 EPB3L1 |
| Gene Full Name | solute carrier family 4 member 2 |
| Gene Summary | This gene encodes a member of the anion exchanger family of membrane transport proteins. The encoded protein regulates intracellular pH, biliary bicarbonate secretion, and chloride uptake. Reduced expression of this gene may be associated with primary biliary cirrhosis (PBC) in human patients, while differential expression of this gene may be associated with malignant hepatocellular carcinoma, colon and gastric cancers. [provided by RefSeq, Nov 2016] |
| Alternative Names | AE 2 AE2 anion exchanger Anion exchange protein 2 Anion exchanger 2 B3A2_HUMAN BAND 3-LIKE PROTEIN BND3L EPB3L1 erythrocyte band 3 Erythrocyte membrane protein band 3 like 1 HKB3 MPB3L NBND3 Non-erythroid band 3-like protein Slc4a2 Solute carrier family 4 member 2 |
| Molecular Weight(MW) | 137 kDa; 137kD(Calculated). |
| Cellular Localization | Membrane>Multi-pass membrane protein. |
WB
Western blot analysis of extracts from A549 cells(UV treatment), using AE2 Antibody. The lane on the left was treated with blocking peptide.
WB
AE2 was up-regulated in human ovarian cancer tissues. (A) AE2 mRNA was over-expressed in ovarian cancer tissues compared to the normal tissues in TCGA dataset. Box-whiskers plot (Min to Max) was shown. (B) Genomic alterations of AE1 (SLC4A1), AE2 (SLC4A2) and AE3 (SLC4A3) in cBioportal ovarian cancer database. (C) mRNA expression of AE2 was examined by qPCR and normalized to GAPDH expression in 100 pairs of matched ovarian cancer tissues and adjacent non-tumorous tissue. Box-whiskers plot (Min to Max) was shown. (D) The protein levels of AE2 were higher in 10 pairs of matched samples of ovarian cancer tissue (T), compared with adjacent non-tumorous tissues (N). GAPDH was served as a loading control. (E) Kaplan–Meier survival analysis of 100 patients with ovarian cancer.| Application Notes | WB:1:1000-3000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
