WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with GLT25D1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with GLT25D1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with GLT25D1 Antibody at dilution of 1:600 incubated at 4 degree celsius over night.IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using GLT25D1 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using GLT25D1 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-GLT25D1 (4ug; Detection:1:500) with HeLa cells lysate 4000ug.ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GLT25D1 antibody at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescent analysis of SH-SY5Y cells using GLT25D1 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | GLT25D1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human GLT25D1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COLGALT1 |
|---|---|
| Gene Synonyms | BSVD3 GLT25D1 ColGalT 1 |
| Gene Full Name | collagen beta(1-O)galactosyltransferase 1 |
| Gene Summary | The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum. [provided by RefSeq, Dec 2015] |
| Alternative Names | PSEC0241 EC:2.4.1.50 Collagen beta(1-O)galactosyltransferase 1 COLGALT1 ColGalT 1 |
| Molecular Weight(MW) | 72kDa |
| Cellular Localization | Endoplasmic reticulum. |
WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with GLT25D1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with GLT25D1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with GLT25D1 Antibody at dilution of 1:600 incubated at 4 degree celsius over night.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using GLT25D1 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using GLT25D1 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-GLT25D1 (4ug; Detection:1:500) with HeLa cells lysate 4000ug.
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GLT25D1 antibody at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescent analysis of SH-SY5Y cells using GLT25D1 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
