WB
Western blot analysis of SULT2B1 using anti-SULT2B1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat testis tissue lysates, Lane 2: A431 whole cell lysates, Lane 3: HELA whole cell lysates, Lane 4: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 41 kDa.ICC/IF
ICC/IF analysis of SULT2B1 using anti-SULT2B1 antibody. SULT2B1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-SULT2B1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).IHC
IHC analysis of SULT2B1 using anti-SULT2B1 antibody. SULT2B1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SULT2B1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of SULT2B1 using anti-SULT2B1 antibody. SULT2B1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SULT2B1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of A549 cells using anti-SULT2B1 antibody. Overlay histogram showing A549 cells stained with PB0867 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SULT2B1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-SULT2B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SULT2B1 different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SULT2B1 |
|---|---|
| Gene Synonyms | HSST2 ARCI14 |
| Gene Full Name | sulfotransferase family 2B member 1 |
| Gene Summary | Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Alternative Names | sulfotransferase family 2B member 1 ARCI14 HSST2 SULT2B1 |
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum,Microsome,Nucleus. |
WB
Western blot analysis of SULT2B1 using anti-SULT2B1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat testis tissue lysates, Lane 2: A431 whole cell lysates, Lane 3: HELA whole cell lysates, Lane 4: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 41 kDa.
ICC/IF
ICC/IF analysis of SULT2B1 using anti-SULT2B1 antibody. SULT2B1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-SULT2B1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
IHC
IHC analysis of SULT2B1 using anti-SULT2B1 antibody. SULT2B1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SULT2B1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of SULT2B1 using anti-SULT2B1 antibody. SULT2B1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SULT2B1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of A549 cells using anti-SULT2B1 antibody. Overlay histogram showing A549 cells stained with PB0867 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SULT2B1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
