WB
Western blot analysis of extracts from various samples, using LEG4 Antibody. Lane 1: Rat heart, blocked with antigen-specific peptides. Lane 2: Rat heart. Lane 3: Mouse spleen. Lane 4: Mcf7 cells(serum starvation treatment). Lane 5: P19 cells(uv treatment).IHC
LEG4 Antibody at 1/100 staining human colorectal cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.IHC
LEG4 Antibody at 1/100 staining human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.ICC/IF
LEG4 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab(#T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | LEG4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human LEG4, corresponding to a region within N-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LGALS4 |
|---|---|
| Gene Synonyms | GAL4 L36LBP |
| Gene Full Name | galectin 4 |
| Gene Summary | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq, Jul 2008] |
| Alternative Names | Antigen NY CO 27 Antigen NY-CO-27 Antigen NYCO27 GAL 4 Gal-4 GAL4 Galectin 4 Galectin-4 Galectin4 Homo sapiens galectin4 mRNA complete cds L 36 lactose binding protein L-36 lactose-binding protein L36 lactose binding protein L36LBP Lactose binding lectin 4 Lactose-binding lectin 4 Lectin galactoside binding soluble 4 LEG4_HUMAN LGALS4 |
| Molecular Weight(MW) | 35 kDa; 36kD(Calculated). |
WB
Western blot analysis of extracts from various samples, using LEG4 Antibody. Lane 1: Rat heart, blocked with antigen-specific peptides. Lane 2: Rat heart. Lane 3: Mouse spleen. Lane 4: Mcf7 cells(serum starvation treatment). Lane 5: P19 cells(uv treatment).
IHC
LEG4 Antibody at 1/100 staining human colorectal cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
IHC
LEG4 Antibody at 1/100 staining human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
ICC/IF
LEG4 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab(#T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:1000-3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
