WB
Western blot analysis of JAK1 expression in NIH/3T3 (A), C6 (B), A-431 (C), MCF7 (D) and HeLa (E) whole cell lysates.IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and cytoplasmic localization.ICC/IF
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.| Product Name | JAK1 Antibody(A-9) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JAK1 |
|---|---|
| Gene Synonyms | JTK3 AIIDE JAK1A JAK1B |
| Gene Full Name | Janus kinase 1 |
| Gene Summary | This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta, interferon-gamma, and cytokine signal transduction. This gene plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. This gene is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune and inflammation response and is a therapeutic target for alleviating cytokine storms. The kinase activity of this gene is directly inhibited by the suppressor of cytokine signalling 1 (SOCS1) protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2020] |
| Molecular Weight(MW) | 130 kDa |
WB
Western blot analysis of JAK1 expression in NIH/3T3 (A), C6 (B), A-431 (C), MCF7 (D) and HeLa (E) whole cell lysates.
IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and cytoplasmic localization.
ICC/IF
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 50 µg IgG1 light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
