WB
Various lysates were subjected to SDS PAGE followed by western blot with BHLHE40 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IP
IP result of anti-BHLHE40 with HeLa cells lysate 1800 ug.IHC
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using BHLHE40 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using BHLHE40 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | BHLHE40 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human BHLHE40 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.65mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BHLHE40 |
|---|---|
| Gene Synonyms | DEC1 HLHB2 BHLHB2 Clast5 SHARP2 STRA13 Stra14 SHARP-2 |
| Gene Full Name | basic helix-loop-helix family member e40 |
| Gene Summary | This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014] |
| Alternative Names | DEC1 bHLHb2 Class B basic helix-loop-helix protein 2 Class E basic helix-loop-helix protein 40 Enhancer-of-split and hairy-related protein 2 |
| Molecular Weight(MW) | 46 kDa(Observed Molecular Weight 46-50 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with BHLHE40 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP
IP result of anti-BHLHE40 with HeLa cells lysate 1800 ug.
IHC
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using BHLHE40 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using BHLHE40 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
