WB
Various lysates were subjected to SDS PAGE followed by western blot with RRM2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.WB
WB result of RRM2 antibody (1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRM2 transfected HepG2 cells.IP
IP result of anti-RRM2 (IP:4ug; Detection:1:2000) with HEK-293 cells lysate 1470 ug.IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using RRM2 antibody at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM2 antibody at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).| Product Name | RRM2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RRM2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:5000-1:50000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:2000-1:8000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RRM2 |
|---|---|
| Gene Synonyms | R2 RR2 RR2M C2orf48 |
| Gene Full Name | ribonucleotide reductase regulatory subunit M2 |
| Gene Summary | This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009] |
| Alternative Names | 2A9A7 EC:1.17.4.1 R2 RR2 RR2M |
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with RRM2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB
WB result of RRM2 antibody (1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRM2 transfected HepG2 cells.
IP
IP result of anti-RRM2 (IP:4ug; Detection:1:2000) with HEK-293 cells lysate 1470 ug.
IHC
Immunohistochemical analysis of paraffin-embedded skin cancer slide using RRM2 antibody at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM2 antibody at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).| Application Notes | WB:1:5000-1:50000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:2000-1:8000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
