WB
human kidney tissue were subjected to SDS PAGE followed by western blot with Endostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver using Endostatin antibody at dilution of 1:50 (under 40x lens).IF-F
Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody. Dilution 1:200. Glutamine synthetase dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Collagen Type XVIII antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | COL18A1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:200-1:1000 IHC:1:20-1:200 IF-P:1:50-1:500 IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseZebrafish |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL18A1 |
|---|---|
| Gene Synonyms | KS KNO GLCC KNO1 |
| Gene Full Name | collagen type XVIII alpha 1 chain |
| Gene Summary | This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | COL18 Collagen Type XVIII Endostatin Collagen alpha 1(XVIII) chain Collagen alpha-1(XVIII) chain |
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Basement membrane,Extracellular matrix,Secreted. |
WB
human kidney tissue were subjected to SDS PAGE followed by western blot with Endostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver using Endostatin antibody at dilution of 1:50 (under 40x lens).
IF-F
Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody. Dilution 1:200. Glutamine synthetase dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Collagen Type XVIII antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:200-1:1000 IHC:1:20-1:200 IF-P:1:50-1:500 IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
