WB
Positive WB detected in: Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg), 786-O whole cell lysate(30µg), U251 whole cell lysate(30µg), CACO2 whole cell lysate(30µg), A431 whole cell lysate(30µg),U87 whole cell lysate(30µg),Mouse Brain tissue lysate(30µg),Rat Brain tissue lysate(30µg), All lanes: ERGIC2 antibody at 1:1000, Goat polyclonal to rabbit IgG at 1/40000 dilution Exposure time: 120s.IHC
IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibodyIHC
IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibodyIHC
IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody| Product Name | ERGIC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from human ERGIC2. AA range:138-200 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ERGIC2 |
|---|---|
| Gene Synonyms | PTX1 CDA14 Erv41 cd002 |
| Gene Full Name | ERGIC and golgi 2 |
| Gene Summary | ERGIC2, or PTX1, is a ubiquitously expressed nuclear protein that is downregulated in prostate carcinoma (Kwok et al., 2001 [PubMed 11445006]).[supplied by OMIM, Aug 2008] |
| Alternative Names | ERGIC2 ERV41 PTX1 CDA14 Endoplasmic reticulum-Golgi intermediate compartment protein 2 |
| Molecular Weight(MW) | 43kDa |
| Cellular Localization | Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm. Nucleus. |
WB
Positive WB detected in: Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg), 786-O whole cell lysate(30µg), U251 whole cell lysate(30µg), CACO2 whole cell lysate(30µg), A431 whole cell lysate(30µg),U87 whole cell lysate(30µg),Mouse Brain tissue lysate(30µg),Rat Brain tissue lysate(30µg), All lanes: ERGIC2 antibody at 1:1000, Goat polyclonal to rabbit IgG at 1/40000 dilution Exposure time: 120s.IHC
IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibodyIHC
IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibodyIHC
IHC image of CSB-PA857020EA01HU diluted at 1:100 and staining in paraffin-embedded human Lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
