WB
Western blot analysis of ERGIC2 using anti-ERGIC2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERGIC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for ERGIC2 at approximately 45 kDa. The expected band size for ERGIC2 is at 43 kDa.IHC
IHC analysis of ERGIC2 using anti-ERGIC2 antibody. ERGIC2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-ERGIC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ERGIC2 using anti-ERGIC2 antibody. ERGIC2 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-ERGIC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of ERGIC2 using anti-ERGIC2 antibody and anti-Alpha Tubulin antibody. ERGIC2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ERGIC2 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.IP
IP analysis of ERGIC2 using anti-ERGIC2 antibody in Hela whole cell lysate. Western blot analysis of ERGIC2 using anti- ERGIC2 antibody. Lane 1: Hela whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- ERGIC2 antibody in Hela whole cell lysate, Lane 3: anti- ERGIC2 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ERGIC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for ERGIC2 at approximately 45 kDa. The expected band size for ERGIC2 is at 43 kDa.| Product Name | Anti-ERGIC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ERGIC2 recombinant protein (Position: E63-H377). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-400 IP:1:250-300 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERGIC2 |
|---|---|
| Gene Synonyms | PTX1 CDA14 Erv41 cd002 |
| Gene Full Name | ERGIC and golgi 2 |
| Gene Summary | ERGIC2, or PTX1, is a ubiquitously expressed nuclear protein that is downregulated in prostate carcinoma (Kwok et al., 2001 [PubMed 11445006]).[supplied by OMIM, Aug 2008] |
| Alternative Names | PTX1 ERV41 ERGIC and golgi 2 Endoplasmic reticulum-Golgi intermediate compartment protein 2 CDA14 |
| Molecular Weight(MW) | 43 kDa |
WB
Western blot analysis of ERGIC2 using anti-ERGIC2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERGIC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for ERGIC2 at approximately 45 kDa. The expected band size for ERGIC2 is at 43 kDa.
IHC
IHC analysis of ERGIC2 using anti-ERGIC2 antibody. ERGIC2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-ERGIC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ERGIC2 using anti-ERGIC2 antibody. ERGIC2 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-ERGIC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ERGIC2 using anti-ERGIC2 antibody and anti-Alpha Tubulin antibody. ERGIC2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ERGIC2 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
IP
IP analysis of ERGIC2 using anti-ERGIC2 antibody in Hela whole cell lysate. Western blot analysis of ERGIC2 using anti- ERGIC2 antibody. Lane 1: Hela whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- ERGIC2 antibody in Hela whole cell lysate, Lane 3: anti- ERGIC2 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ERGIC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for ERGIC2 at approximately 45 kDa. The expected band size for ERGIC2 is at 43 kDa.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-400 IP:1:250-300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
