WB
Western blot analysis of CD45RA expression in Raji (A), Ramos (B) and U-937 (C) whole cell lysatesIHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing membrane and cytoplasmic staining of cells in germinal centers and cells in nongerminal centers.| Product Name | CD45RA Mouse Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against human hairy cell leukaemia cells. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:1000-1:10000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPRC |
|---|---|
| Gene Synonyms | LCA LY5 B220 CD45 L-CA T200 CD45R GP180 IMD105 |
| Gene Full Name | protein tyrosine phosphatase receptor type C |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
WB
Western blot analysis of CD45RA expression in Raji (A), Ramos (B) and U-937 (C) whole cell lysates
IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing membrane and cytoplasmic staining of cells in germinal centers and cells in nongerminal centers.| Application Notes | WB:1:1000-1:10000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 50 µg IgG2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
