WB
Various lysates were subjected to SDS PAGE followed by western blot with TRAP1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using TRAP1 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (Clone: 1H5D4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).IP
IP result of anti-TRAP1 (IP:5ug; Detection:1:1000) with K-562 cells lysate 1760 ug.| Product Name | TRAP1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TRAP1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000-1:10000 IHC:1:250-1:1000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 1.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRAP1 |
|---|---|
| Gene Synonyms | HSP75 HSP 75 HSP90L TRAP-1 |
| Gene Full Name | TNF receptor associated protein 1 |
| Gene Summary | This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
| Alternative Names | TNFR associated protein 1 HSPC5 HSP90L HSP75 HSP 75 |
| Molecular Weight(MW) | 80kDa(Observed MW: 70-75 kDakDa) |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with TRAP1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using TRAP1 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (Clone: 1H5D4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IP
IP result of anti-TRAP1 (IP:5ug; Detection:1:1000) with K-562 cells lysate 1760 ug.| Application Notes | WB:1:2000-1:10000 IHC:1:250-1:1000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
