WB
Western blot analysis of anti-TRX2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRX2 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for TRX2 at approximately 14 kDa. The expected band size for TRX2 is at 14 kDa.IHC
IHC analysis of TRX2 using anti-TRX2 antibody. TRX2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-TRX2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TRX2 using anti-TRX2 antibody. TRX2 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-TRX2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-TRX2/TXN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Thioredoxin 2 Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-2000 IHC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TXN2 |
|---|---|
| Gene Synonyms | TXN MTRX TRX2 MT-TRX COXPD29 |
| Gene Full Name | thioredoxin 2 |
| Gene Summary | This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008] |
| Alternative Names | MT TRX MTRX Thioredoxin 2 Thioredoxin mitochondrial TRX2 TXN2 |
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Mitochondrion |
WB
Western blot analysis of anti-TRX2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRX2 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for TRX2 at approximately 14 kDa. The expected band size for TRX2 is at 14 kDa.
IHC
IHC analysis of TRX2 using anti-TRX2 antibody. TRX2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-TRX2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TRX2 using anti-TRX2 antibody. TRX2 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-TRX2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-2000 IHC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
