WB
Western blot analysis of TRX2/TXN2 using anti-TRX2/TXN2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: Raji whole cell lysates, Lane 3: K562 whole cell lysates, Lane 4: A549 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: THP-1 whole cell lysates, Lane 7: PC-12 whole cell lysates, Lane 8: RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-TRX2/TXN2 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for TRX2/TXN2 at approximately 14 kDa. The expected band size for TRX2/TXN2 is at 14 kDa.ICC/IF
ICC/IF analysis of TRX2/TXN2 using anti-TRX2/TXN2 antibody. TRX2/TXN2 was detected in an immunocytochemical section of Hela cells. The section was incubated with mouse anti-TRX2/TXN2 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of TXN2 using anti-TXN2 antibody.TXN2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-TXN2 Antibody overnight at 4°C. Fluoro488 Conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.FC
Flow Cytometry analysis of HL-60 cells using anti-TRX2/TXN2 antibody. Overlay histogram showing HL-60 cells stained with M04586-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TRX2/TXN2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.IHC
IHC analysis of TRX2/TXN2 using anti-TRX2/TXN2 antibody. TRX2/TXN2 was detected in a paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-TRX2/TXN2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Product Name | Anti-TRX2/TXN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Thioredoxin 2/TXN2 recombinant protein (Position: T60-G166). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 ICC/IF: FC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A/G |
| Gene Symbol | TXN2 |
|---|---|
| Gene Synonyms | TXN MTRX TRX2 MT-TRX COXPD29 |
| Gene Full Name | thioredoxin 2 |
| Gene Summary | This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008] |
| Alternative Names | MT TRX MTRX Thioredoxin 2 Thioredoxin mitochondrial TRX2 TXN2 |
| Molecular Weight(MW) | 14 kDa(Observed MW 14kDa) |
| Cellular Localization | Mitochondrion |
WB
Western blot analysis of TRX2/TXN2 using anti-TRX2/TXN2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: Raji whole cell lysates, Lane 3: K562 whole cell lysates, Lane 4: A549 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: THP-1 whole cell lysates, Lane 7: PC-12 whole cell lysates, Lane 8: RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-TRX2/TXN2 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for TRX2/TXN2 at approximately 14 kDa. The expected band size for TRX2/TXN2 is at 14 kDa.
ICC/IF
ICC/IF analysis of TRX2/TXN2 using anti-TRX2/TXN2 antibody. TRX2/TXN2 was detected in an immunocytochemical section of Hela cells. The section was incubated with mouse anti-TRX2/TXN2 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of TXN2 using anti-TXN2 antibody.TXN2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-TXN2 Antibody overnight at 4°C. Fluoro488 Conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FC
Flow Cytometry analysis of HL-60 cells using anti-TRX2/TXN2 antibody. Overlay histogram showing HL-60 cells stained with M04586-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TRX2/TXN2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IHC
IHC analysis of TRX2/TXN2 using anti-TRX2/TXN2 antibody. TRX2/TXN2 was detected in a paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-TRX2/TXN2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Application Notes | WB:1:500-2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 ICC/IF: FC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
