WB
25 ug total protein per lane of various lysates (see on figure) probed with CRIM1 polyclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Product Name | CRIM1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CRIM1 : 901-1028/1028 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Crim1 |
|---|---|
| Gene Full Name | cysteine rich transmembrane BMP regulator 1 |
| Gene Summary | Predicted to enable PDZ domain binding activity. Predicted to act upstream of or within negative regulation of BMP signaling pathway. Predicted to be located in extracellular region and membrane. Is expressed in several structures, including central nervous system; genitourinary system; heart; integumental system; and sensory organ. Orthologous to human CRIM1 (cysteine rich transmembrane BMP regulator 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CRIM-1 S52 CRIM1_HUMAN CRIM1 Cysteine-rich repeat-containing protein S52 CRIM1_MOUSE cysteine rich transmembrane BMP regulator 1 cysteine-rich motor neuron 1 cysteine rich transmembrane BMP regulator 1 (chordin-like) |
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Secreted and Cell membrane. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with CRIM1 polyclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
