WB
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-SMPD4 at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilutionIHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMPD4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Product Name | SMPD4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human SMPD4 : 201-300/827 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-2000 IHC:1:100-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMPD4 |
|---|---|
| Gene Synonyms | SKNY NET13 NEDMABA NEDMEBA NSMASE3 NSMASE-3 |
| Gene Full Name | sphingomyelin phosphodiesterase 4 |
| Gene Summary | The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017] |
| Alternative Names | NEDMABA NEDMEBA NET13 NSMASE-3 NSMASE3 SKNY 4122402O22Rik RGD1310674 NSMA3_HUMAN SMPD4 Neutral sphingomyelinase 3 (nSMase-3 | nSMase3) Neutral sphingomyelinase III 3.1.4.12 KIAA1418 NSMA3_MOUSE sphingomyelin phosphodiesterase 4 sphingomyelin phosphodiesterase 4 neutral membrane (neutral sphingomyelinase-3) neutral sphingomyelinase-3 |
| Molecular Weight(MW) | 93 kDa(Observed band size: 96 kD) |
| Cellular Localization | Endoplasmic reticulum membrane. Golgi apparatus membrane. |
WB
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-SMPD4 at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution
IHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMPD4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Application Notes | WB:1:500-2000 IHC:1:100-500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
