WB
Western blot analysis of RPS28 using anti-RPS28 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPS28 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for RPS28 at approximately 12 kDa. The expected band size for RPS28 is at 8 kDa.IHC
IHC analysis of RPS28 using anti-RPS28 antibody. RPS28 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-RPS28 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of RPS28 using anti-RPS28 antibody. RPS28 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with rabbit anti-RPS28 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of RPS28 using anti-RPS28 antibody and anti-Beta Tubulin antibody. RPS28 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-RPS28 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)were used as secondary antibody.| Product Name | Anti-RPS28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human RPS28 recombinant protein (Position: M1-R69). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMonkeyRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS28 |
|---|---|
| Gene Synonyms | S28 eS28 DBA15 |
| Gene Full Name | ribosomal protein S28 |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S28E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Alternative Names | 40S ribosomal protein S28 ribosomal protein S28 RPS28 |
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum,Nucleus. |
WB
Western blot analysis of RPS28 using anti-RPS28 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPS28 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for RPS28 at approximately 12 kDa. The expected band size for RPS28 is at 8 kDa.
IHC
IHC analysis of RPS28 using anti-RPS28 antibody. RPS28 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-RPS28 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of RPS28 using anti-RPS28 antibody. RPS28 was detected in a paraffin-embedded section of human endometrial cancer tissue. The tissue section was incubated with rabbit anti-RPS28 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of RPS28 using anti-RPS28 antibody and anti-Beta Tubulin antibody. RPS28 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-RPS28 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)were used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
