IHC
Immunohistochemistry of paraffin-embedded human testis tissue using GAK Antibody at dilution of 1:100ICC/IF
Immunofluorescence staining of HepG2 cells with GAK Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | GAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Cyclin-G-associated kinase protein (939-1086AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | GAK |
|---|---|
| Gene Synonyms | DNAJ26 DNAJC26 |
| Gene Full Name | cyclin G associated kinase |
| Gene Summary | In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| Alternative Names | Auxilin 2 antibody Cyclin G associated kinase antibody Cyclin-G-associated kinase antibody DNAJ26 antibody DNAJC26 antibody EC 2.7.11.1 antibody FLJ16629 antibody FLJ40395 antibody GAK antibody GAK protein antibody GAK_HUMAN antibody MGC99654 antibody |
| Cellular Localization | Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. |
IHC
Immunohistochemistry of paraffin-embedded human testis tissue using GAK Antibody at dilution of 1:100
ICC/IF
Immunofluorescence staining of HepG2 cells with GAK Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
