WB
Western blot analysis of DCBLD2 using anti-DCBLD2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DCBLD2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for DCBLD2 at approximately 120 kDa. The expected band size for DCBLD2 is at 85 kDa.IHC
IHC analysis of DCBLD2 using anti-DCBLD2 antibody . DCBLD2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-DCBLD2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of DCBLD2 using anti-DCBLD2 antibody . DCBLD2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-DCBLD2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of DCBLD2 using anti-DCBLD2 antibody in U251 whole cell lysate. Western blot analysis of DCBLD2 using anti- DCBLD2 antibody. Lane 1: U251 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- DCBLD2 antibody in U251 whole cell lysate, Lane 3: anti- DCBLD2 antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- DCBLD2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for DCBLD2 at approximately 120 kDa. The expected band size for DCBLD2 is at 85 kDa.| Product Name | Anti-DCBLD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human DCBLD2 recombinant protein (Position: E102-G761). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCBLD2 |
|---|---|
| Gene Synonyms | ESDN CLCP1 |
| Gene Full Name | discoidin, CUB and LCCL domain containing 2 |
| Gene Summary | Predicted to enable signaling receptor activity. Involved in negative regulation of cell growth and wound healing. Located in cell surface and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ESDN Endothelial and smooth muscle cell-derived neuropilin-like protein Discoidin CUB and LCCL domain-containing protein 2 CUB LCCL and coagulation factor V/VIII-homology domains protein 1 CLCP1 |
| Molecular Weight(MW) | 120 kDa(Observed MW 85 kDa) |
| Cellular Localization | Membrane |
WB
Western blot analysis of DCBLD2 using anti-DCBLD2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DCBLD2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for DCBLD2 at approximately 120 kDa. The expected band size for DCBLD2 is at 85 kDa.
IHC
IHC analysis of DCBLD2 using anti-DCBLD2 antibody . DCBLD2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-DCBLD2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of DCBLD2 using anti-DCBLD2 antibody . DCBLD2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-DCBLD2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of DCBLD2 using anti-DCBLD2 antibody in U251 whole cell lysate. Western blot analysis of DCBLD2 using anti- DCBLD2 antibody. Lane 1: U251 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- DCBLD2 antibody in U251 whole cell lysate, Lane 3: anti- DCBLD2 antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- DCBLD2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for DCBLD2 at approximately 120 kDa. The expected band size for DCBLD2 is at 85 kDa.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
