WB
Western blot analysis of TGN46/TGOLN2 using anti-TGN46/TGOLN2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGN46/TGOLN2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for TGN46/TGOLN2 at approximately 90-100 kDa. The expected band size for TGN46/TGOLN2 is at 50 kDa.IHC
IHC analysis of TGN46/TGOLN2 using anti-TGN46/TGOLN2 antibody. TGN46/TGOLN2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-TGN46/TGOLN2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of TGN46/TGOLN2 using anti-TGN46/TGOLN2 antibody. TGN46/TGOLN2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-TGN46/TGOLN2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U937 cells using anti-TGN46/TGOLN2 antibody. Overlay histogram showing U937 cells stained with A06591-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGN46/TGOLN2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-TGN46/TGOLN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human TGN46/TGOLN2 recombinant protein (Position: E319-K436). Human TGN46/TGOLN2 shares 78% and 77.1% amino acid (aa) sequence identity with mouse and rat TGN46/TGOLN2, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TGOLN2 |
|---|---|
| Gene Synonyms | TGN38 TGN46 TGN48 TGN51 TTGN2 hTGN46 hTGN48 hTGN51 |
| Gene Full Name | trans-golgi network protein 2 |
| Gene Summary | This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | TGOLN2 TGN48 TGN38 homolog TGN38 hTGN51 |
| Molecular Weight(MW) | 50kDa |
| Cellular Localization | Cell membrane,Golgi apparatus,Membrane |
WB
Western blot analysis of TGN46/TGOLN2 using anti-TGN46/TGOLN2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGN46/TGOLN2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for TGN46/TGOLN2 at approximately 90-100 kDa. The expected band size for TGN46/TGOLN2 is at 50 kDa.
IHC
IHC analysis of TGN46/TGOLN2 using anti-TGN46/TGOLN2 antibody. TGN46/TGOLN2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-TGN46/TGOLN2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of TGN46/TGOLN2 using anti-TGN46/TGOLN2 antibody. TGN46/TGOLN2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-TGN46/TGOLN2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U937 cells using anti-TGN46/TGOLN2 antibody. Overlay histogram showing U937 cells stained with A06591-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGN46/TGOLN2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
